Site-directed spin labeling of proteins for distance measurements in vitro and in cells

Org Biomol Chem. 2016 Jun 15;14(24):5468-76. doi: 10.1039/c6ob00473c.


Site-directed spin labeling (SDSL) in combination with electron paramagnetic resonance (EPR) spectroscopy allows studying the structure, dynamics, and interactions of proteins via distance measurements in the nanometer range. We here give an overview of available spin labels, the strategies for their introduction into proteins, and the associated potentials for protein structural studies in vitro and in the context of living cells.

Publication types

  • Review

MeSH terms

  • Electron Spin Resonance Spectroscopy
  • Escherichia coli / chemistry*
  • Escherichia coli / cytology
  • Protein Conformation
  • Proteins / chemistry*
  • Spin Labels


  • Proteins
  • Spin Labels