Citrus flavanones prevent systemic inflammation and ameliorate oxidative stress in C57BL/6J mice fed high-fat diet

Food Funct. 2016 Jun 15;7(6):2675-81. doi: 10.1039/c5fo01541c. Epub 2016 May 16.


The flavanones hesperidin, eriocitrin and eriodictyol were investigated for their prevention of the oxidative stress and systemic inflammation caused by high-fat diet in C57BL/6J mice. The mice received a standard diet (9.5% kcal from fat), high-fat diet (45% kcal from fat) or high-fat diet supplemented with hesperidin, eriocitrin or eriodictyol for a period of four weeks. Hesperidin, eriocitrin and eriodictyol increased the serum total antioxidant capacity, and restrained the elevation of interleukin-6 (IL-6), macrophage chemoattractant protein-1 (MCP-1), and C-reactive protein (hs-CRP). In addition, the liver TBARS levels and spleen mass (g per kg body weight) were lower for the flavanone-treated mice than in the unsupplemented mice. Eriocitrin and eriodictyol reduced TBARS levels in the blood serum, and hesperidin and eriodictyol also reduced fat accumulation and liver damage. The results showed that hesperidin, eriocitrin and eriodictyol had protective effects against inflammation and oxidative stress caused by high-fat diet in mice, and may therefore prevent metabolic alterations associated with the development of cardiovascular diseases in other animals.

MeSH terms

  • Animals
  • Blood Glucose / metabolism
  • C-Reactive Protein / metabolism
  • Chemokine CCL2 / blood
  • Chemotactic Factors / blood
  • Cholesterol / blood
  • Citrus / chemistry*
  • Cytokines / blood
  • Diet, High-Fat / adverse effects
  • Flavanones / pharmacology*
  • Hesperidin / pharmacology
  • Inflammation / drug therapy*
  • Liver / drug effects
  • Liver / metabolism
  • Macrophages / drug effects
  • Macrophages / metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Oxidative Stress / drug effects*
  • Protective Agents / analysis
  • Protective Agents / pharmacology
  • Spleen / drug effects
  • Spleen / metabolism
  • Thiobarbituric Acid Reactive Substances / metabolism
  • Triglycerides / blood


  • Blood Glucose
  • Ccl2 protein, mouse
  • Chemokine CCL2
  • Chemotactic Factors
  • Cytokines
  • Flavanones
  • Protective Agents
  • Thiobarbituric Acid Reactive Substances
  • Triglycerides
  • C-Reactive Protein
  • Cholesterol
  • eriocitrin
  • Hesperidin
  • eriodictyol