Single-Reaction Multiplex Reverse Transcription PCR for Detection of Zika, Chikungunya, and Dengue Viruses

Emerg Infect Dis. 2016 Jul;22(7):1295-7. doi: 10.3201/eid2207.160326. Epub 2016 Jul 15.


Clinical manifestations of Zika virus, chikungunya virus, and dengue virus infections can be similar. To improve virus detection, streamline molecular workflow, and decrease test costs, we developed and evaluated a multiplex real-time reverse transcription PCR for these viruses.

Keywords: Zika virus; arboviruses; chikungunya virus; dengue virus; multiplex molecular diagnostics; real-time RT-PCR; vector-borne infections; viruses.

MeSH terms

  • Chikungunya virus / isolation & purification*
  • Dengue Virus / isolation & purification*
  • Genome, Viral
  • Humans
  • Multiplex Polymerase Chain Reaction / methods*
  • RNA, Viral / genetics
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Zika Virus / isolation & purification*


  • RNA, Viral