A Flow SPR Immunosensor Based on a Sandwich Direct Method

Mauro Tomassetti; Giorgia Conta; Luigi Campanella; Gabriele Favero; Gabriella Sanzò; Franco Mazzei; Riccarda Antiochia

doi:10.3390/bios6020022

A Flow SPR Immunosensor Based on a Sandwich Direct Method

Biosensors (Basel). 2016 May 13;6(2):22. doi: 10.3390/bios6020022.

Authors

Mauro Tomassetti¹, Giorgia Conta², Luigi Campanella³, Gabriele Favero⁴, Gabriella Sanzò⁵, Franco Mazzei⁶, Riccarda Antiochia⁷

Affiliations

¹ Department of Chemistry, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy. Mauro.tomassetti@uniroma1.it.
² Department of Chemistry, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy. giorgia.conta@libero.it.
³ Department of Chemistry, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy. luigi.campanella@uniroma1.it.
⁴ Department of Chemistry and Drug Technologies, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy. gabriele.favero@uniroma1.it.
⁵ Department of Chemistry and Drug Technologies, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy. gabriella.sanzo@uniroma1.it.
⁶ Department of Chemistry and Drug Technologies, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy. franco.mazzei@uniroma1.it.
⁷ Department of Chemistry and Drug Technologies, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy. riccarda.antiochia@uniroma1.it.

Abstract

In this study, we report the development of an SPR (Surface Plasmon Resonance) immunosensor for the detection of ampicillin, operating under flow conditions. SPR sensors based on both direct (with the immobilization of the antibody) and competitive (with the immobilization of the antigen) methods did not allow the detection of ampicillin. Therefore, a sandwich-based sensor was developed which showed a good linear response towards ampicillin between 10(-3) and 10(-1) M, a measurement time of ≤20 min and a high selectivity both towards β-lactam antibiotics and antibiotics of different classes.

Keywords: ampicillin; flow immunosensor SPR; sandwich method.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Ampicillin
Anti-Bacterial Agents
Biosensing Techniques*
Immunoassay*
Sensitivity and Specificity
Surface Plasmon Resonance* / instrumentation
Surface Plasmon Resonance* / methods

Substances

Anti-Bacterial Agents
Ampicillin