The effect of red grape seed extract on serum paraoxonase activity in patients with mild to moderate hyperlipidemia

Sao Paulo Med J. 2016 May 13;134(3):234-9. doi: 10.1590/1516-3180.2015.01702312.


Context and objective: Red grape seed extract (RGSE) contains oligomeric proanthocyanidin complexes as a class of flavonoids. These compounds are potent antioxidants and exert many health-promoting effects. This study aimed to determine the effects of RGSE on serum levels of triglycerides (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), apolipoprotein AI (apo-AI) levels and paraoxonase (PON) activity in patients with mild to moderate hyperlipidemia (MMH).

Design and settings: A randomized double-blind placebo-controlled clinical trial was conducted at Shahid-Modarres Hospital (Tehran, Iran) and Tabriz University of Medical Sciences. Seventy MMH patients were randomly assigned to receive treatment (200 mg/day of RGSE) or placebo for eight weeks.

Results: Significant elevation in serum levels of apo-AI (P = 0.001), HDL-C (P = 0.001) and PON activity (P = 0.001) and marked decreases in concentrations of TC (P = 0.015), TG (P = 0.011) and LDL-C (P = 0.014) were found in the cases. PON activity was significantly correlated with apo-AI (r = 0.270; P < 0.01) and HDL-C (r = 0.45; P < 0.001). Significant differences between the RGSE and control groups (before and after treatment) for TC (P = 0.001), TG (P = 0.001), PON (P = 0.03), apo-AI (P = 0.001) and LDL-C (P = 0.002) were seen.

Conclusion: It is possible that RGSE increases PON activity mostly through increasing HDL-C and apo-AI levels in MMH patients. It may thus have potential beneficial effects in preventing oxidative stress and atherosclerosis in these patients.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Adult
  • Antioxidants / therapeutic use*
  • Aryldialkylphosphatase / blood*
  • Cholesterol / blood
  • Double-Blind Method
  • Female
  • Grape Seed Extract / therapeutic use*
  • Humans
  • Hyperlipidemias / drug therapy*
  • Lipoproteins, HDL / blood
  • Lipoproteins, LDL / blood
  • Male
  • Middle Aged
  • Phytotherapy
  • Placebos
  • Triglycerides / blood
  • Young Adult


  • Antioxidants
  • Grape Seed Extract
  • Lipoproteins, HDL
  • Lipoproteins, LDL
  • Placebos
  • Triglycerides
  • Cholesterol
  • Aryldialkylphosphatase