Short-term ubiquinol supplementation reduces oxidative stress associated with strenuous exercise in healthy adults: A randomized trial

Biofactors. 2016 Nov 12;42(6):612-622. doi: 10.1002/biof.1297. Epub 2016 May 19.


Studies about Coenzyme Q10 (CoQ10 ) supplementation on strenuous exercise are scarce, especially those related with oxidative stress associated with physical activity and virtually nonexistent with the reduced form, Ubiquinol. The objective of this study was to determine, for the first time, whether a short-term supplementation with Ubiquinol can prevent oxidative stress associated to strenuous exercise. The participants (n = 100 healthy and well trained, but not on an elite level) were classified in two groups: Ubiquinol (experimental group), and placebo group (control). The protocol consisted of conducting two identical strenuous exercise tests with a rest period between tests of 24 h. Blood and urine samples were collected from the participants before supplementation (basal value) (T1), after supplementation (2 weeks) (T2), after first physical exercise test (T3), after 24 h of rest (T4), and after second physical exercise test (T5).The increase observed in the lactate, isoprostanes, DNA damage, and hydroperoxide levels reveals the severity of the oxidative damage induced by the exercise. There was a reduction in the isoprostanes, 8-OHdG, oxidized LDL, and hydroperoxydes in the supplemented Ubiquinol group, an increase in total antioxidant status, fat soluble antioxidant (both plasma and membrane), and CAT activity. Also, NO in the Ubiquinol-supplemented group was maintained within a narrow range. Oxidative stress induced by strenuous exercise is accumulative and increases transiently in subsequent sessions of physical activity. A short-term supplementation (2 weeks) with Ubiquinol (200 mg/day) before strenuous exercise, decreases oxidative stress and increases plasma NO, fact that could improve endothelial function, energetic substrate supply, and muscle recovery after strenuous exercise. © 2016 BioFactors, 42(6):612-622, 2016.

Keywords: Ubiquinol; high intensity (strenuous) exercise; oxidative damage.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Adult
  • Antioxidants / administration & dosage*
  • Dietary Supplements
  • Drug Administration Schedule
  • Healthy Volunteers
  • Humans
  • Male
  • Middle Aged
  • Oxidative Stress / drug effects*
  • Physical Exertion / drug effects*
  • Resistance Training
  • Ubiquinone / administration & dosage
  • Ubiquinone / analogs & derivatives*


  • Antioxidants
  • Ubiquinone
  • ubiquinol