N-acyl dopamines induce cell death in PC12 cell line via induction of nitric oxide generation and oxidative stress

A M Ashba; M G Akimov; N M Gretskaya; V V Bezuglov

doi:10.1134/S1607672916020010

N-acyl dopamines induce cell death in PC12 cell line via induction of nitric oxide generation and oxidative stress

Dokl Biochem Biophys. 2016 Mar;467(1):81-4. doi: 10.1134/S1607672916020010. Epub 2016 May 20.

Authors

A M Ashba¹, M G Akimov², N M Gretskaya¹, V V Bezuglov¹

Affiliations

¹ Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia.
² Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia. akimovmike@yandex.ru.

PMID: 27193703
DOI: 10.1134/S1607672916020010

Abstract

It was shown that dopamine amides of arachidonic, oleic, and docosahexaenoic acids exhibit toxicity with respect to PC12 pheochromocytoma cell line. The mechanism of realization of the cytotoxic effect of acyl dopamines is the induction of oxidative stress. This event is preceded by triggering the synthesis of nitric oxide.

MeSH terms

Animals
Cell Death / drug effects*
Cell Death / physiology
Cell Survival / drug effects
Cell Survival / physiology
Dopamine / analogs & derivatives*
Dopamine / toxicity
Dose-Response Relationship, Drug
Enzyme Inhibitors / pharmacology
Fatty Acids, Unsaturated / toxicity*
NG-Nitroarginine Methyl Ester / pharmacology
Nitric Oxide / metabolism*
Nitric Oxide Synthase / antagonists & inhibitors
Nitric Oxide Synthase / metabolism
Oxidative Stress / drug effects*
Oxidative Stress / physiology
PC12 Cells
Rats
Reactive Oxygen Species / metabolism

Substances

Enzyme Inhibitors
Fatty Acids, Unsaturated
Reactive Oxygen Species
Nitric Oxide
Nitric Oxide Synthase
NG-Nitroarginine Methyl Ester
Dopamine