Studying Kinetochore Kinases

Methods Mol Biol. 2016;1413:333-47. doi: 10.1007/978-1-4939-3542-0_21.

Abstract

Mitotic kinetochores are signaling network hubs that regulate chromosome movements, attachment error-correction, and the spindle assembly checkpoint. Key switches in these networks are kinases and phosphatases that enable rapid responses to changing conditions. Describing the mechanisms and dynamics of their localized activation and deactivation is therefore instrumental for understanding the spatiotemporal control of chromosome segregation.

Keywords: Antibody; Kinase; Kinetochore; Phosphorylation; Signaling; Small molecule inhibitor; Spindle checkpoint.

MeSH terms

  • Enzyme Activation
  • Kinetochores / metabolism*
  • Mitosis
  • Molecular Imaging / methods
  • Phosphorylation
  • Phosphotransferases / antagonists & inhibitors
  • Phosphotransferases / metabolism*
  • Protein Kinase Inhibitors / pharmacology
  • Protein Transport
  • Signal Transduction
  • Spindle Apparatus / metabolism
  • Time-Lapse Imaging

Substances

  • Protein Kinase Inhibitors
  • Phosphotransferases