Variation of the nucleotide and encoded amino acid sequences of the envelope gene from eight dengue-2 viruses

Arch Virol. 1989;105(1-2):39-53. doi: 10.1007/BF01311115.

Abstract

The nucleotide sequences of the envelope genes from five Thai and three Sri Lankan dengue-2 viruses were determined by sequencing the viral RNA using synthetic oligonucleotide primers. The results were compared with the four published dengue-2 envelope sequences to obtain a classification of these viruses, which showed that the Thai isolates could be divided into two separate groups while the Sri Lankan isolates were distinct. There was no correlation between disease severity and envelope protein sequence, or between year of isolation and sequence. No particular amino acid changes were associated with virulence or a change in hydrophilic region which could perhaps act as an epitope.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Dengue / microbiology
  • Dengue Virus / classification
  • Dengue Virus / genetics*
  • Dengue Virus / pathogenicity
  • Genes, Viral
  • Humans
  • Molecular Sequence Data
  • Oligonucleotides / chemical synthesis
  • Oligonucleotides / genetics
  • Phylogeny
  • RNA, Viral
  • Serotyping
  • Species Specificity
  • Sri Lanka
  • Thailand
  • Viral Envelope Proteins / genetics*
  • Virulence

Substances

  • Oligonucleotides
  • RNA, Viral
  • Viral Envelope Proteins

Associated data

  • GENBANK/M24444
  • GENBANK/M24445
  • GENBANK/M24446
  • GENBANK/M24447
  • GENBANK/M24448
  • GENBANK/M24449
  • GENBANK/M24450
  • GENBANK/M24451