Molecular analysis for bacterial contamination in dental unit water lines

New Microbiol. 2016 Apr;39(2):143-5.

Abstract

Bacterial contamination in dental unit waterlines (DUWLs) was evaluated by molecular techniques in addition to the conventional culture method. Water samples (n=8) from DUWLs were investigated for heterotrophic bacteria by culture method using R2A agar. The selected bacterial antibiotic-resistance genes and Legionella species-specific 16SrDNA were identified by PCR. The profiles of bacterial contamination in DUWLs were further identified by PCR-DGGE. In this study, no antibiotic-resistant or Legionella genes were detected. Polycyclic aromatic hydrocarbon-degrading bacterium, Novosphingobium sp. was the most prevalent in DUWLs. Conventional PCR and PCR-DGGE were shown to be potentially useful for monitoring of bacterial contamination in DUWLs.

Keywords: Bacterial contamination; Dental unit water line; Molecular analysis; PCR-DGGE.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Bacteria / classification
  • Bacteria / isolation & purification*
  • Bacterial Load
  • Bacteriological Techniques
  • DNA, Bacterial / genetics
  • DNA, Bacterial / isolation & purification*
  • Dental Equipment / microbiology*
  • Dentistry
  • Drug Resistance, Bacterial
  • Equipment Contamination
  • Humans
  • Polymerase Chain Reaction / methods
  • RNA, Bacterial / genetics
  • RNA, Ribosomal, 16S / genetics
  • Water Microbiology*
  • Water Supply / standards*

Substances

  • Anti-Bacterial Agents
  • DNA, Bacterial
  • RNA, Bacterial
  • RNA, Ribosomal, 16S