DNA sequencing with chain-terminating inhibitors

Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7. doi: 10.1073/pnas.74.12.5463.


A new method for determining nucleotide sequences in DNA is described. It is similar to the "plus and minus" method [Sanger, F. & Coulson, A. R. (1975) J. Mol. Biol. 94, 441-448] but makes use of the 2',3'-dideoxy and arabinonucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase. The technique has been applied to the DNA of bacteriophage varphiX174 and is more rapid and more accurate than either the plus or the minus method.

MeSH terms

  • Base Sequence*
  • Coliphages / analysis
  • DNA Polymerase I / antagonists & inhibitors
  • DNA Restriction Enzymes / metabolism
  • DNA, Viral*
  • Deoxyribonucleotides / pharmacology
  • Methods


  • DNA, Viral
  • Deoxyribonucleotides
  • DNA Polymerase I
  • DNA Restriction Enzymes

Associated data

  • GENBANK/M10849
  • GENBANK/M10860
  • GENBANK/M10861
  • GENBANK/M10862