Passive Immune-Protection of Litopenaeus vannamei against Vibrio harveyi and Vibrio parahaemolyticus Infections with Anti-Vibrio Egg Yolk (IgY)-Encapsulated Feed

Int J Mol Sci. 2016 May 17;17(5):723. doi: 10.3390/ijms17050723.

Abstract

Vibrio spp. are major causes of mortality in white shrimp (Litopenaeus vannamei) which is lacking adaptive immunity. Passive immunization with a specific egg yolk antibody (IgY) is a potential method for the protection of shrimp against vibriosis. In this study, immune effects of the specific egg yolk powders (IgY) against both V. harveyi and V. parahaemolyticus on white shrimp were evaluated. The egg yolk powders against V. harveyi and V. parahaemolyticus for passive immunization of white shrimp were prepared, while a tube agglutination assay and an indirect enzyme-linked immunosorbent assay (ELISA) were used for detection of IgY titer. Anti-Vibrio egg yolk was encapsulated by β-cyclodextrin, which could keep the activity of the antibody in the gastrointestinal tract of shrimp. The results showed that the anti-Vibrio egg powders had an inhibiting effect on V. harveyi and V. parahaemolyticus in vitro. Lower mortality of infected zoeae, mysis, and postlarva was observed in groups fed with anti-Vibrio egg powders, compared with those fed with normal egg powders. The bacterial load in postlarva fed with specific egg powders in seeding ponds was significantly lower than those fed with normal egg powders in seeding ponds. These results show that passive immunization by oral administration with specific egg yolk powders (IgY) may provide a valuable protection of vibrio infections in white shrimp.

Keywords: IgY; Litopenaeus vannamei; Vibrio harveyi; Vibrio parahaemolyticus; passive immunization.

MeSH terms

  • Animals
  • Bacterial Load / drug effects
  • Egg Yolk / immunology
  • Immunization, Passive / methods
  • Immunoglobulins / pharmacology*
  • Larva / drug effects
  • Larva / microbiology
  • Penaeidae / drug effects
  • Penaeidae / immunology*
  • Penaeidae / microbiology
  • Vibrio / drug effects*
  • Vibrio / physiology
  • Vibrio Infections / prevention & control*
  • Vibrio parahaemolyticus / drug effects
  • Vibrio parahaemolyticus / physiology
  • beta-Cyclodextrins / chemistry

Substances

  • IgY
  • Immunoglobulins
  • beta-Cyclodextrins