A novel ratiometric two-photon fluorescent probe for imaging of Pd(2+) ions in living cells and tissues

Liyi Zhou; Shunqin Hu; Haifei Wang; Hongyan Sun; Xiaobing Zhang

doi:10.1016/j.saa.2016.05.013

A novel ratiometric two-photon fluorescent probe for imaging of Pd(2+) ions in living cells and tissues

Spectrochim Acta A Mol Biomol Spectrosc. 2016 Sep 5:166:25-30. doi: 10.1016/j.saa.2016.05.013. Epub 2016 May 12.

Authors

Liyi Zhou¹, Shunqin Hu², Haifei Wang², Hongyan Sun³, Xiaobing Zhang⁴

Affiliations

¹ College of Packaging and Materials Engineering, Hunan University of Technology, Hunan 412007, PR China; Molecular Sciences and Biomedicine Laboratory, State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University, Changsha 410082, PR China; Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, PR China. Electronic address: zhouly0817@163.com.
² College of Packaging and Materials Engineering, Hunan University of Technology, Hunan 412007, PR China.
³ Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, PR China.
⁴ Molecular Sciences and Biomedicine Laboratory, State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University, Changsha 410082, PR China.

PMID: 27203231
DOI: 10.1016/j.saa.2016.05.013

Abstract

Ratiometric two-photon fluorescent probes can not only eliminate interferences from environmental factors but also achieve deep-tissue imaging with improved spatial localization. To quantitatively track Pd(2+) in biosystems, herein, we reported a ratiometric two-photon fluorescent probe, termed as Np-Pd, which based on a D-π-A-structure two-photon fluorophore of the naphthalimide derivative and deprotection of aryl propargyl ethers by palladium species. The probe Np-Pd displayed a more than 25-fold enhancement towards palladium species with high sensitivity and selectivity. Additionally, the probe Np-Pd was further used for fluorescence imaging of Pd(2+) ions in living cells and tissues under two-photon excitation (820nm), which showed large tissue-imaging depth (19.6-184.6μm), and a high resolution for ratiometric imaging.

Keywords: Fluorescent imaging; Naphthalimide derivative; Palladium; Ratiometric fluorescent probe; Two-photon.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cations, Divalent / analysis
Fluorescence
Fluorescent Dyes / chemistry*
HeLa Cells
Humans
Liver / chemistry
Mice
Microscopy, Fluorescence / methods
Naphthalimides / chemistry*
Optical Imaging / methods*
Palladium / analysis*
Photons

Substances

Cations, Divalent
Fluorescent Dyes
Naphthalimides
Palladium