High-throughput production of a stable isotope-labeled peptide library for targeted proteomics using a wheat germ cell-free synthesis system

Mol Biosyst. 2016 Jul 19;12(8):2389-93. doi: 10.1039/c6mb00209a.

Abstract

Quantitative proteomic approaches using selected reaction monitoring (SRM) are currently limited by the difficulty in the preparation of reference standards. In this study, we demonstrat the high-throughput production of a reference peptide library using a wheat germ cell-free synthesis system to develop a large-scale SRM assay for targeted proteomics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell-Free System
  • High-Throughput Screening Assays*
  • Isotope Labeling*
  • Peptide Library*
  • Proteomics* / methods
  • Seeds / metabolism*
  • Tandem Mass Spectrometry
  • Triticum / metabolism*

Substances

  • Peptide Library