ChIP-seq reveals broad roles of SARD1 and CBP60g in regulating plant immunity

Nat Commun. 2015 Dec 18;6:10159. doi: 10.1038/ncomms10159.

Abstract

Recognition of pathogens by host plants leads to rapid transcriptional reprogramming and activation of defence responses. The expression of many defence regulators is induced in this process, but the mechanisms of how they are controlled transcriptionally are largely unknown. Here we use chromatin immunoprecipitation sequencing to show that the transcription factors SARD1 and CBP60g bind to the promoter regions of a large number of genes encoding key regulators of plant immunity. Among them are positive regulators of systemic immunity and signalling components for effector-triggered immunity and PAMP-triggered immunity, which is consistent with the critical roles of SARD1 and CBP60g in these processes. In addition, SARD1 and CBP60g target a number of genes encoding negative regulators of plant immunity, suggesting that they are also involved in negative feedback regulation of defence responses. Based on these findings we propose that SARD1 and CBP60g function as master regulators of plant immune responses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / genetics*
  • Arabidopsis / immunology
  • Arabidopsis / microbiology
  • Arabidopsis Proteins / genetics*
  • Arabidopsis Proteins / immunology
  • Base Sequence
  • Binding Sites
  • Calmodulin-Binding Proteins / genetics*
  • Calmodulin-Binding Proteins / immunology
  • Chromatin Immunoprecipitation
  • Feedback, Physiological
  • Gene Expression Regulation, Plant*
  • High-Throughput Nucleotide Sequencing
  • Mutation
  • Plant Diseases / genetics*
  • Plant Diseases / immunology
  • Plant Diseases / microbiology
  • Plant Immunity / genetics*
  • Promoter Regions, Genetic
  • Protein Binding
  • Pseudomonas syringae / pathogenicity
  • Pseudomonas syringae / physiology
  • Signal Transduction
  • Transcription, Genetic

Substances

  • Arabidopsis Proteins
  • CBP60g protein, Arabidopsis
  • Calmodulin-Binding Proteins
  • SARD1 protein, Arabidopsis