Identification of human RNA editing sites: A historical perspective

Gokul Ramaswami; Jin Billy Li

doi:10.1016/j.ymeth.2016.05.011

Identification of human RNA editing sites: A historical perspective

Methods. 2016 Sep 1:107:42-7. doi: 10.1016/j.ymeth.2016.05.011. Epub 2016 May 18.

Authors

Gokul Ramaswami¹, Jin Billy Li²

Affiliations

¹ Department of Genetics, Stanford University, Stanford, CA 94305, USA; Program in Neurogenetics, Department of Neurology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA. Electronic address: GRamaswami@mednet.ucla.edu.
² Department of Genetics, Stanford University, Stanford, CA 94305, USA. Electronic address: jin.billy.li@stanford.edu.

Abstract

A-to-I RNA editing is an essential gene regulatory mechanism. Once thought to be a rare phenomenon only occurring in a few transcripts, the emergence of high-throughput RNA sequencing has facilitated the identification of over 2 million RNA editing sites in the human transcriptome. In this review, we survey the current RNA-seq based methods as well as historical methods used to identify RNA editing sites.

Keywords: Post-transcriptional gene regulation; RNA editing; RNA sequencing.

Publication types

Review
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Genome, Human / genetics*
High-Throughput Nucleotide Sequencing / methods*
Humans
RNA Editing / genetics*
Sequence Analysis, RNA
Transcriptome / genetics*

Grants and funding

R01 GM102484/GM/NIGMS NIH HHS/United States