Polycrystalline structures formed in evaporating droplets as a parameter to test the action of Zincum metallicum 30c in a wheat seed model

Homeopathy. 2016 May;105(2):173-9. doi: 10.1016/j.homp.2015.10.002. Epub 2015 Nov 12.


Background: Polycrystalline structures formed inside evaporating droplets of different biological fluids have been shown sensitive towards various influences, including ultra high dilutions (UHDs), representing so a new approach potentially useful for basic research in homeopathy. In the present study we tested on a wheat seed model Zincum metallicum 30c efficacy versus lactose 30c and water.

Materials and methods: Stressed and non-stressed wheat seeds were watered with the three treatments. Seed-leakage droplets were evaporated and the polycrystalline structures formed inside the droplet residues were analyzed for their local connected fractal dimensions (LCFDs) (measure of complexity) using the software ImageJ.

Results: We have found significant differences in LCFD values of polycrystalline structures obtained from stressed seeds following the treatments (p<0.0001); Zincum metallicum 30c lowered the structures' complexity compared to lactose 30c and water. In non-stressed seeds no significant differences were found.

Conclusions: The droplet evaporation method (DEM) might represent a potentially useful tool in basic research in homeopathy. Furthermore our results suggest a sensitization of the stressed model towards the treatment action, which is conforming to previous findings.

Keywords: Crystallization; Droplet evaporation method (DEM); Ultra high dilutions (UHDs); Wheat seeds; Zincum metallicum.

Publication types

  • Evaluation Study

MeSH terms

  • Chemistry Techniques, Analytical
  • Crystallization
  • Germination / drug effects*
  • Homeopathy*
  • Humans
  • Models, Biological
  • Seeds / drug effects
  • Triticum*
  • Zinc / pharmacology*


  • Zinc