Depth and time-dependent heterogeneity of microglia in mouse hippocampal slice cultures

Neurosci Res. 2016 Oct;111:64-9. doi: 10.1016/j.neures.2016.05.001. Epub 2016 May 17.


Microglia are the brain-resident immune cells with the phagocytic capacity to engulf dead and living neurons in health and disease. However, the mechanisms underlying the neuron-microglia interaction remain elusive partly because proper in vitro systems are lacking. Specifically, the highly activated status of microglia with amoeboid morphology in primary culture is different from the 'resting' microglia with multiple processes in vivo. Here, we performed a detailed investigation of microglial properties in mouse hippocampal slice cultures, focusing on the changes in morphology in the activated state, finding a depth and time-dependent localization of in vivo-like microglia in slice cultures.

Keywords: Hippocampus; Microglia; Microglial heterogeneity; Mouse; Slice culture.

MeSH terms

  • Animals
  • Hippocampus / cytology*
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Microglia / cytology*
  • Neurons / cytology
  • Time Factors
  • Tissue Culture Techniques