Multi-Omics of Single Cells: Strategies and Applications

Trends Biotechnol. 2016 Aug;34(8):605-608. doi: 10.1016/j.tibtech.2016.04.004. Epub 2016 May 20.

Abstract

Most genome-wide assays provide averages across large numbers of cells, but recent technological advances promise to overcome this limitation. Pioneering single-cell assays are now available for genome, epigenome, transcriptome, proteome, and metabolome profiling. Here, we describe how these different dimensions can be combined into multi-omics assays that provide comprehensive profiles of the same cell.

Keywords: bioinformatic methods; cell state profiling; combined genome/epigenome/transcriptome/proteome/metabolome mapping; molecular medicine; single-cell analysis; single-cell systems biology.

MeSH terms

  • Cell Physiological Phenomena*
  • Gene Expression Profiling / methods
  • Gene Expression Profiling / trends*
  • Genomics / trends*
  • High-Throughput Screening Assays / methods
  • High-Throughput Screening Assays / trends*
  • Systems Integration
  • Tissue Array Analysis / methods
  • Tissue Array Analysis / trends*