Characterization of microsatellites identified by next-generation sequencing in the Neotropical tree Handroanthus billbergii (Bignoniaceae)

Eduardo Morillo; Johanna Buitron; Ricardo Limongi; Helene Vignes; Xavier Argout

doi:10.3732/apps.1500135

Characterization of microsatellites identified by next-generation sequencing in the Neotropical tree Handroanthus billbergii (Bignoniaceae)

Appl Plant Sci. 2016 May 9;4(5):apps.1500135. doi: 10.3732/apps.1500135. eCollection 2016 May.

Authors

Eduardo Morillo¹, Johanna Buitron¹, Ricardo Limongi¹, Helene Vignes², Xavier Argout²

Affiliations

¹ Instituto Nacional de Investigaciones Agropecuarias (INIAP), Casilla Postal 17-01-340, Quito, Ecuador.
² Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), UMR 1334 AGAP, Montpellier, France.

Abstract

Premise of the study: We developed microsatellite (simple sequence repeat [SSR]) markers in the Neotropical tree Handroanthus billbergii (Bignoniaceae), to be applied in assessment of genetic diversity in this species as a reference for inferring the impact of dry forest fragmentation in Ecuador.

Methods and results: Using next-generation sequencing, we detected a total of 26,893 putative SSRs reported here. Using an ABI 3500xl sequencer, we identified and characterized a set of polymorphic markers in 23 individuals belonging to three populations of H. billbergii.

Conclusions: We report a set of 30 useful SSR markers for H. billbergii and a large list of potential microsatellites for developing new markers for this or related species.

Keywords: Bignoniaceae; Handroanthus billbergii; microsatellites; next-generation sequencing (NGS).