Genome Sequence of Canine Herpesvirus

PLoS One. 2016 May 23;11(5):e0156015. doi: 10.1371/journal.pone.0156015. eCollection 2016.


Canine herpesvirus is a widespread alphaherpesvirus that causes a fatal haemorrhagic disease of neonatal puppies. We have used high-throughput methods to determine the genome sequences of three viral strains (0194, V777 and V1154) isolated in the United Kingdom between 1985 and 2000. The sequences are very closely related to each other. The canine herpesvirus genome is estimated to be 125 kbp in size and consists of a unique long sequence (97.5 kbp) and a unique short sequence (7.7 kbp) that are each flanked by terminal and internal inverted repeats (38 bp and 10.0 kbp, respectively). The overall nucleotide composition is 31.6% G+C, which is the lowest among the completely sequenced alphaherpesviruses. The genome contains 76 open reading frames predicted to encode functional proteins, all of which have counterparts in other alphaherpesviruses. The availability of the sequences will facilitate future research on the diagnosis and treatment of canine herpesvirus-associated disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alphaherpesvirinae / genetics*
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chromosome Mapping
  • Dog Diseases / virology*
  • Dogs
  • Genes, Viral
  • Genome, Viral*
  • Hemorrhagic Disorders / veterinary
  • Hemorrhagic Disorders / virology
  • Madin Darby Canine Kidney Cells
  • Open Reading Frames
  • Sequence Analysis, DNA
  • Viral Proteins / genetics


  • Viral Proteins