Association of PTPN22 rs2476601 Polymorphism with Rheumatoid Arthritis and Celiac Disease in Khuzestan Province, Southwestern Iran

Iran Biomed J. 2017 Jan;21(1):61-6. doi: 10.6091/.21.1.61. Epub 2016 May 24.

Abstract

Background: Single-nucleotide polymorphism (SNP) rs2476601 within protein tyrosine phosphatase non-receptor type 22 gene (PTPN22) has been shown to be a risk factor for different autoimmune diseases. This study explored the association of 1858 C/T SNP with rheumatoid arthritis (RA) and celiac disease (CD) in a region covering south-west of Iran.

Methods: Totally, 52 patients with CD, 120 patients with RA, and 120 healthy subjects were selected. The samples were genotyped for the rs2476601 in PTPN22 gene using the tetra-amplification refractory mutation system polymerase chain reaction.

Results: The frequency of +1858T risk allele was significantly increased in both RA (P=0.021, OR=2.56, 95%CI=1.19-5.47) and CD (P=0.002, OR=3.87, 95%CI=1.68-8.95) patients, as compared to the control group. However, no association was found between the +1858C/T PTPN22 gene SNP and the anti-cyclic citrullinated peptide and rheumatoid factor positivity in RA patients.

Conclusions: PTPN22 gene could play a crucial role in people's susceptibility to certain autoimmune diseases.

Keywords: Celiac disease; PTPN22; Rheumatoid arthritis; Rheumatoid factor.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Arthritis, Rheumatoid / genetics*
  • Autoantibodies / blood*
  • Autoantibodies / immunology
  • Celiac Disease / genetics*
  • Child
  • Female
  • Gene Frequency / genetics
  • Genetic Predisposition to Disease*
  • Humans
  • Iran
  • Male
  • Middle Aged
  • Peptides, Cyclic / immunology
  • Polymorphism, Single Nucleotide / genetics
  • Protein Tyrosine Phosphatase, Non-Receptor Type 22 / genetics*
  • Rheumatoid Factor / blood
  • Young Adult

Substances

  • Autoantibodies
  • Peptides, Cyclic
  • cyclic citrullinated peptide
  • Rheumatoid Factor
  • PTPN22 protein, human
  • Protein Tyrosine Phosphatase, Non-Receptor Type 22