Differences in ozone sensitivity among woody species are related to leaf morphology and antioxidant levels

Tree Physiol. 2016 Sep;36(9):1105-16. doi: 10.1093/treephys/tpw042. Epub 2016 May 22.


Ozone (O3) sensitivity varies greatly among plant species. Leaf traits such as stomatal conductance, antioxidant capacity and leaf morphology and anatomy may play important roles in controlling this variation, but the relative contributions of each trait remain elusive. In this study, we examined the differences in O3 sensitivity among 29 deciduous and evergreen woody species used for urban greening in China in an open-top chamber experiment. Elevated O3 caused visible injury and reductions in net photosynthesis, and these effects differed significantly among species. The deciduous species Sorbaria sorbifolia, Hibiscus syriacus and Fraxinus chinensis were the most sensitive, while evergreen species ranked among the most tolerant. O3 sensitivity was linked to both low leaf mass per area (LMA) and low leaf area-based antioxidant levels, but not to variation in leaf mass-based antioxidant levels or stomatal conductance. The well-known and easily measured leaf trait LMA thus represents a potentially useful metric for O3 risk assessment and for selecting appropriate species for urban greening in O3-polluted areas.

Keywords: antioxidant defense; gas exchange; leaf mass per area; urban greening species; visible symptoms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / metabolism
  • China
  • Ozone / pharmacology*
  • Plant Leaves / anatomy & histology
  • Plant Leaves / drug effects*
  • Plant Leaves / metabolism
  • Plants / drug effects*
  • Plants / metabolism
  • Species Specificity
  • Trees / drug effects
  • Trees / metabolism


  • Antioxidants
  • Ozone