Endothelial function following glucose ingestion in adults with prediabetes: Role of exercise intensity

Obesity (Silver Spring). 2016 Jul;24(7):1515-21. doi: 10.1002/oby.21522. Epub 2016 May 25.


Objective: To determine whether high intensity exercise (HIE) would improve endothelial function more than an isocaloric bout of moderate intensity exercise (MIE) following glucose ingestion in adults with prediabetes.

Methods: Twelve subjects with prediabetes completed all three conditions: time-course matched control and isocaloric exercise (∼200 kcal) at moderate (MIE; at lactate threshold) and high intensity (HIE; 75% of difference between lactate threshold and VO2 peak). Brachial artery flow-mediated dilation (FMD) was measured before exercise (baseline), within 30 min postexercise, and 1 and 2 hr following a 75 g oral glucose tolerance test (OGTT). Plasma F2-isoprostanes were also assessed during the protocol (i.e., baseline to 2 hr OGTT) as a biomarker of oxidative stress.

Results: MIE reduced postexercise F2-isoprostanesAUC compared with time-course matched control and HIE. Although exercise had no statistical effect on FMD postexercise or during the OGTT, elevations in FMDAUC after MIE and HIE were associated with reduced postexercise F2-isoprostanesAUC .

Conclusions: Exercise at either intensity had no effect on FMD immediately postexercise following glucose administration. However, individuals with reduced oxidative stress responses to exercise had greater exercise-induced improvement in FMD. Further work is required to identify the mechanism by which exercise alters oxidative stress to enhance endothelial function.

MeSH terms

  • Brachial Artery / physiopathology
  • Endothelium, Vascular / physiology*
  • Exercise / physiology*
  • Exercise Tolerance / drug effects
  • F2-Isoprostanes / blood
  • Female
  • Glucose / pharmacology*
  • Glucose Tolerance Test
  • Humans
  • Male
  • Middle Aged
  • Oxidative Stress / physiology
  • Prediabetic State / drug therapy
  • Prediabetic State / physiopathology*
  • Sweetening Agents / pharmacology*


  • F2-Isoprostanes
  • Sweetening Agents
  • Glucose