Sedimentitalea todarodis sp. nov., isolated from the intestinal tract of a Japanese flying squid

Int J Syst Evol Microbiol. 2016 Sep;66(9):3293-3298. doi: 10.1099/ijsem.0.001188. Epub 2016 May 25.

Abstract

A novel Gram-stain-negative, motile, aerobic and rod-shaped alphaproteobacterium, designated strain KHS03T, was isolated from the intestinal tract of a Japanese flying squid, Todarodes pacificus, which was collected from the East Sea, Korea. The new isolate shared 97.4 % 16S rRNA gene sequence similarity with Sedimentitalea nanhaiensis NH52FT. The isolate grew optimally at 25 °C and pH 7 in the presence of 1-2 % (w/v) NaCl, and had an absolute requirement of sodium ions for growth. The major cellular fatty acid was C18 : 1ω7c. The primary isoprenoid quinone was ubiquinone-10. Polar lipids comprised diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminolipid and two unidentified lipids. Genotypic analyses indicated that the whole genomic DNA of the isolate had a G+C content of 59.9 mol%. DNA-DNA hybridization showed that the isolate shared 17.1±2.3 % (reciprocal 17.0±1.9 %) genomic relatedness with the closest related type strain, S. nanhaiensis NH52FT. Strain KHS03T is thus suggested to represent a novel species of the genus Sedimentitalea, for which the name Sedimentitalea todarodis sp. nov. is proposed. The type strain is KHS03T (=KCTC 42412T=JCM 31160T).

MeSH terms

  • Animals
  • Bacterial Typing Techniques
  • Base Composition
  • DNA, Bacterial / genetics
  • Decapodiformes / microbiology*
  • Fatty Acids / chemistry
  • Intestines / microbiology*
  • Nucleic Acid Hybridization
  • Phospholipids / chemistry
  • Phylogeny*
  • Republic of Korea
  • Rhodobacteraceae / classification*
  • Rhodobacteraceae / genetics
  • Rhodobacteraceae / isolation & purification
  • Sequence Analysis, DNA
  • Ubiquinone / chemistry

Substances

  • DNA, Bacterial
  • Fatty Acids
  • Phospholipids
  • Ubiquinone
  • Ubiquinone Q2