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A Novel Polymorphic Repeat in the Upstream Regulatory Region of the Estrogen-Induced Gene EIG121 Is Not Associated With the Risk of Developing Breast or Endometrial Cancer

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A Novel Polymorphic Repeat in the Upstream Regulatory Region of the Estrogen-Induced Gene EIG121 Is Not Associated With the Risk of Developing Breast or Endometrial Cancer

Katherine A Bolton et al. BMC Res Notes.

Abstract

Background: The estrogen-induced gene 121 (EIG121) has been associated with breast and endometrial cancers, but its mechanism of action remains unknown. In a genome-wide search for tandem repeats, we found that EIG121 contains a short tandem repeat (STR) in its upstream regulatory region which has the potential to alter gene expression. The presence of this STR has not previously been analysed in relation to breast or endometrial cancer risk.

Results: In this study, the lengths of this STR were determined by PCR, fragment analysis and sequencing using DNA from 223 breast cancer patients, 204 endometrial cancer patients and 220 healthy controls to determine if they were associated with the risk of developing breast or endometrial cancer. We found this repeat to be highly variable with the number of copies of the AG motif ranging from 27 to 72 and having a bimodal distribution. No statistically significant association was identified between the length of this STR and the risk of developing breast or endometrial cancer or age at diagnosis.

Conclusions: The STR in the upstream regulatory region of EIG121 is highly polymorphic, but is not associated with the risk of developing breast or endometrial cancer in the cohorts analysed here. While this polymorphic STR in the regulatory region of EIG121 appears to have no impact on the risk of developing breast or endometrial cancer, its association with disease recurrence or overall survival remains to be determined.

Keywords: Breast cancer; Cancer risk; EIG121; Endometrial cancer; KIAA1324; Microsatellites; Regulatory region; STR; Short tandem repeats.

Figures

Fig. 1
Fig. 1
Histogram showing the bimodal distribution of EIG121 STR lengths across all three cohorts (breast cancer, endometrial cancer and healthy control samples)

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References

    1. Brookes KJ. The VNTR in complex disorders: the forgotten polymorphisms? A functional way forward? Genomics. 2013;101(5):273–281. doi: 10.1016/j.ygeno.2013.03.003. - DOI - PubMed
    1. Hannan AJ. Tandem repeat polymorphisms: modulators of disease susceptibility and candidates for ‘missing heritability’. Trends Genet. 2010;26(2):59–65. doi: 10.1016/j.tig.2009.11.008. - DOI - PubMed
    1. Press MO, Carlson KD, Queitsch C. The overdue promise of short tandem repeat variation for heritability. Trends Genet. 2014;30(11):504–512. doi: 10.1016/j.tig.2014.07.008. - DOI - PMC - PubMed
    1. Bolton KA, Ross JP, Grice DM, Bowden NA, Holliday EG, Avery-Kiejda KA, Scott RJ. STaRRRT: a table of short tandem repeats in regulatory regions of the human genome. BMC Genom. 2013;14(1):795. doi: 10.1186/1471-2164-14-795. - DOI - PMC - PubMed
    1. Westin SN, Broaddus RR, Deng L, McCampbell A, Lu KH, Lacour RA, Milam MR, Urbauer DL, Mueller P, Pickar JH, et al. Molecular clustering of endometrial carcinoma based on estrogen-induced gene expression. Cancer Biol Ther. 2009;8(22):2126–2135. doi: 10.4161/cbt.8.22.9740. - DOI - PMC - PubMed
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