Nuclear-localized cyclic nucleotide-gated channels mediate symbiotic calcium oscillations

Science. 2016 May 27;352(6289):1102-5. doi: 10.1126/science.aae0109.


Nuclear-associated Ca(2+) oscillations mediate plant responses to beneficial microbial partners--namely, nitrogen-fixing rhizobial bacteria that colonize roots of legumes and arbuscular mycorrhizal fungi that colonize roots of the majority of plant species. A potassium-permeable channel is known to be required for symbiotic Ca(2+) oscillations, but the calcium channels themselves have been unknown until now. We show that three cyclic nucleotide-gated channels in Medicago truncatula are required for nuclear Ca(2+) oscillations and subsequent symbiotic responses. These cyclic nucleotide-gated channels are located at the nuclear envelope and are permeable to Ca(2+) We demonstrate that the cyclic nucleotide-gated channels form a complex with the postassium-permeable channel, which modulates nuclear Ca(2+) release. These channels, like their counterparts in animal cells, might regulate multiple nuclear Ca(2+) responses to developmental and environmental conditions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calcium Channels / genetics
  • Calcium Channels / metabolism*
  • Calcium Signaling*
  • Cyclic Nucleotide-Gated Cation Channels / genetics
  • Cyclic Nucleotide-Gated Cation Channels / metabolism*
  • Medicago truncatula / genetics
  • Medicago truncatula / metabolism*
  • Nuclear Envelope / metabolism*
  • Nucleotides, Cyclic / metabolism*
  • Plant Roots / metabolism
  • Potassium Channels / metabolism
  • Symbiosis


  • Calcium Channels
  • Cyclic Nucleotide-Gated Cation Channels
  • Nucleotides, Cyclic
  • Potassium Channels