Intravenous infusion of apolipoprotein E accelerates clearance of plasma lipoproteins in rabbits

J Clin Invest. 1989 Jun;83(6):2125-30. doi: 10.1172/JCI114126.


Plasma cholesterol levels in cholesterol-fed rabbits were markedly reduced by the intravenous infusion or bolus injection of recombinant human apo E or rabbit plasma apo E. Administration of 6-70 mg of apo E resulted in an approximately 20-40% acute reduction in plasma cholesterol levels within 2-3 h. Plasma cholesterol levels remained reduced for 4-8 h after the administration of apo E. Furthermore, the intravenous injection of apo E reduced the plasma cholesterol levels in Watanabe heritable hyperlipidemic rabbits. The addition of apo E to [14C]cholesterol-labeled canine thoracic duct lymph or [14C]cholesterol-labeled chylomicrons resulted in accelerated plasma clearance of these diet-induced lipoproteins in normal rabbits, with the uptake occurring primarily in the liver. This study suggests that the amount or availability of apo E in the plasma of cholesterol-fed rabbits may be rate limiting for the normal clearance of diet-induced remnant lipoproteins.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Apolipoproteins E / blood
  • Apolipoproteins E / pharmacokinetics*
  • Cholesterol, Dietary / administration & dosage
  • Chylomicrons / metabolism
  • Dogs
  • Hypercholesterolemia / blood
  • Infusions, Intravenous
  • Injections, Intravenous
  • Lipoproteins / blood*
  • Male
  • Metabolic Clearance Rate
  • Rabbits
  • Recombinant Proteins / pharmacokinetics
  • Sodium Chloride / administration & dosage


  • Apolipoproteins E
  • Cholesterol, Dietary
  • Chylomicrons
  • Lipoproteins
  • Recombinant Proteins
  • Sodium Chloride