Proteomic analysis of minute amount of colonic biopsies by enteroscopy sampling

Biochem Biophys Res Commun. 2016 Aug 5;476(4):286-292. doi: 10.1016/j.bbrc.2016.05.114. Epub 2016 May 24.


Colorectal cancer (CRC) is one of the most common types of malignant tumor worldwide. Currently, although many researchers have been devoting themselves in CRC studies, the process of locating biomarkers for CRC early diagnosis and prognostic is still very slow. Using a centrifugal proteomic reactor-based proteomic analysis of minute amount of colonic biopsies by enteroscopy sampling, 2620 protein groups were quantified between cancer mucosa and adjacent normal colorectal mucosa. Of which, 403 protein groups were differentially expressed with statistic significance between cancer and normal tissues, including 195 up-regulated and 208 down-regulated proteins in cancer tissues. Three proteins (SOD3, PRELP and NGAL) were selected for further Western blot validation. And the resulting Western blot experimental results were consistent with the quantitative proteomic data. SOD3 and PRELP are down-regulated in CRC mucosa comparing to adjacent normal tissue, while NGAL is up-regulated in CRC mucosa. In conclusion, the centrifugal proteomic reactor-based label-free quantitative proteomic approach provides a highly sensitive and powerful tool for analyzing minute protein sample from tiny colorectal biopsies, which may facilitate CRC biomarkers discovery for diagnoses and prognoses.

Keywords: Centrifugal proteomic reactor; Colorectal cancer; Endoscopy; Label-free proteomics; Minute amount.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers, Tumor / analysis
  • Biomarkers, Tumor / metabolism
  • Colon / metabolism
  • Colon / pathology*
  • Colorectal Neoplasms / diagnosis*
  • Colorectal Neoplasms / metabolism
  • Colorectal Neoplasms / pathology
  • Early Detection of Cancer
  • Endoscopy
  • Extracellular Matrix Proteins / analysis
  • Extracellular Matrix Proteins / metabolism
  • Glycoproteins / analysis
  • Glycoproteins / metabolism
  • Humans
  • Intestinal Mucosa / metabolism
  • Intestinal Mucosa / pathology*
  • Lipocalin-2 / analysis
  • Lipocalin-2 / metabolism
  • Protein Interaction Mapping
  • Proteome / analysis*
  • Proteome / metabolism
  • Proteomics / methods*
  • Rectum / metabolism
  • Rectum / pathology*
  • Sample Size
  • Signal Transduction
  • Superoxide Dismutase / analysis
  • Superoxide Dismutase / metabolism


  • Biomarkers, Tumor
  • Extracellular Matrix Proteins
  • Glycoproteins
  • LCN2 protein, human
  • Lipocalin-2
  • PRELP protein, human
  • Proteome
  • SOD3 protein, human
  • Superoxide Dismutase