Association of gene polymorphisms of FV, FII, MTHFR, SERPINE1, CTLA4, IL10, and TNFalpha with pre-eclampsia in Chinese women

Inflamm Res. 2016 Sep;65(9):717-24. doi: 10.1007/s00011-016-0953-y. Epub 2016 May 27.


Objectives: To investigate the association of polymorphisms in genes involved in coagulation, fibrinolysis, and inflammation with pre-eclampsia (PE) in a Chinese population.

Methods: It is a case-control study of patients with PE (n = 117) and controls (n = 286) from the Maternal and Children's Hospital of Shenzhen City carried out between June 2014 and May 2015. The rs6025, rs6020, rs1801133, rs1799963, rs1799889, rs231775, rs1800896, rs1800629, and rs1799724 polymorphisms were analyzed using Snap Shot. Multifactor dimensionality reduction (MDR) and logistic regression analyses were carried out to assess the interactions among these SNPs.

Results: The frequencies of polymorphisms in tumor necrosis factor-α (TNF-α) (rs1800629 and rs1799724) and interleukin 10 (IL-10) (rs1800896) were significantly different between patients with PE and controls (P < 0.05). The best interaction model identified a marginally significant interaction between rs1799724 and rs1800896 (P = 0.05).

Conclusions: This study suggests that polymorphisms in the TNF-α and IL-10 genes could be associated with PE, but additional studies are necessary to explore the mechanisms involving these polymorphisms and the gene-gene interactions involved in the susceptibility to PE.

Keywords: Interleukin 10; Multifactor dimensionality reduction; Polymorphism; Pre-eclampsia; Tumor necrosis factor-alpha.

MeSH terms

  • Adult
  • Asian Continental Ancestry Group / genetics*
  • CTLA-4 Antigen / genetics
  • Case-Control Studies
  • China / epidemiology
  • Factor V / genetics
  • Female
  • Genetic Predisposition to Disease*
  • Genotype
  • Humans
  • Interleukin-10 / genetics
  • Methylenetetrahydrofolate Reductase (NADPH2) / genetics
  • Plasminogen Activator Inhibitor 1 / genetics
  • Polymorphism, Single Nucleotide
  • Pre-Eclampsia / epidemiology
  • Pre-Eclampsia / genetics*
  • Pregnancy
  • Prothrombin / genetics
  • Tumor Necrosis Factor-alpha / genetics


  • CTLA-4 Antigen
  • CTLA4 protein, human
  • IL10 protein, human
  • Plasminogen Activator Inhibitor 1
  • SERPINE1 protein, human
  • Tumor Necrosis Factor-alpha
  • Interleukin-10
  • Factor V
  • Prothrombin
  • MTHFR protein, human
  • Methylenetetrahydrofolate Reductase (NADPH2)