GMP-Compliant Expansion of Clinical-Grade Human Mesenchymal Stromal/Stem Cells Using a Closed Hollow Fiber Bioreactor

Methods Mol Biol. 2016;1416:389-412. doi: 10.1007/978-1-4939-3584-0_23.

Abstract

This chapter describes a method for GMP-compliant expansion of human mesenchymal stromal/stem cells (hMSC) from bone marrow aspirates, using the Quantum(®) Cell Expansion System from Terumo BCT. The Quantum system is a functionally closed, automated hollow fiber bioreactor system designed to reproducibly grow cells in either GMP or research laboratory environments. The chapter includes protocols for preparation of media, setup of the Quantum system, coating of the hollow fiber bioreactor, as well as loading, feeding, and harvesting of cells. We suggest a panel of quality controls for the starting material, the interim product, as well as the final product.

Keywords: Bioreactor; Clinical application; Functionally closed; GMP; Glucose consumption; Hollow fiber; Lactate generation; MSC; Mesenchymal stem cells; Mesenchymal stromal cells; Platelet lysate.

MeSH terms

  • Bioreactors*
  • Cell Culture Techniques / instrumentation*
  • Cell Differentiation
  • Cell Proliferation
  • Cells, Cultured
  • Culture Media / chemistry
  • Humans
  • Manufactured Materials / standards*
  • Mesenchymal Stem Cells / cytology*
  • Quality Control
  • Quantum Theory

Substances

  • Culture Media