Momordica charantia polysaccharides mitigate the progression of STZ induced diabetic nephropathy in rats

Int J Biol Macromol. 2016 Oct;91:394-9. doi: 10.1016/j.ijbiomac.2016.05.090. Epub 2016 May 26.

Abstract

Diabetic nephropathy (DN) has become a primary cause of end-stage kidney disease. Several complex dynamics converge together to accelerate the advancement of DN. The present investigation was postulated to explore the mechanism of reno-protective nature of Momordica Charantia polysaccharides (MCP) by evaluating the anti-hyperglycemic, anti-lipidemic as well as markers for oxidative stress and antioxidant proficiency in streptozotocin (STZ)-induced diabetic rats. The oral administration of MCP showed a significant normalization in the levels of kidney function test in the STZ-induced diabetic rats. The levels of blood urea nitrogen (BUN), urea protein and creatinine increased by 316.58%, 195.14% and 800.97% respectively, in STZ-induced diabetic rats when compared with normal rats. MCP treatment also illustrated a significant improvement in glutathione peroxidase, superoxide dismutase and catalase levels, with a significant decline in MDA in diabetic kidneys. Immunoblots of heme-oxygenase 1 (HO-1) and Nrf2 of MCP treated diabetic rats showed a significant up-regulation of HO-1 and Nrf2 protein. Histological and ultra-structural observations also reveal that MCP efficiently protects the kidneys from hyperglycemia-mediated oxidative damage. These findings illustrate that the reno-protective nature of MCP mitigates the progression of STZ induced DN in rats by suppression of oxidative stress and amelioration of the HO-1/Nrf2 pathway.

Keywords: Diabetic nepropathy; Momordica charantia; Polysaccharides.

MeSH terms

  • Animals
  • Diabetes Mellitus, Experimental / drug therapy*
  • Diabetes Mellitus, Experimental / metabolism
  • Diabetic Nephropathies / chemically induced
  • Diabetic Nephropathies / drug therapy*
  • Diabetic Nephropathies / metabolism
  • Heme Oxygenase-1 / metabolism
  • Kidney / metabolism
  • Male
  • Momordica charantia / chemistry*
  • NF-E2-Related Factor 2 / metabolism
  • Oxidative Stress / drug effects*
  • Polysaccharides / chemistry
  • Polysaccharides / pharmacology*
  • Rats
  • Signal Transduction / drug effects*

Substances

  • NF-E2-Related Factor 2
  • Nfe2l2 protein, rat
  • Polysaccharides
  • Heme Oxygenase-1