Accelerated Processing of Solitary and Clustered Abasic Site DNA Damage Lesions by APE1 in the Presence of Aqueous Extract of Ganoderma Lucidum

J Biosci. 2016 Jun;41(2):265-75. doi: 10.1007/s12038-016-9614-0.

Abstract

The stimulatory effect of the aqueous extract of G. lucidum, a basidiomycetes class fungus in the APE1-enzyme-mediated processing of solitary and bistranded clustered abasic sites DNA damages is presented. Abasic sites are considered the most common type of DNA damage lesions. Our study shows enhanced activity of APE1 in the processing of abasic sites in the presence of the polysaccharides fraction of G. lucidum. Remarkable increase in the amount of single-strand breaks (SSBs) and double-strand breaks (DSBs) from solitary and bistranded clustered abasic sites respectively with APE1 in the presence of the extract was found. This trend is maintained when abasic sites in DNA oligomers are exposed to fibroblast cell extracts in the presence of the extract. While DNA conformational alteration is negligible, APE1 enzyme shows characteristic changes in the alpha helix and beta strand ratio after incubation with G. lucidum extract. The enhanced reactivity of APE1 at the molecular level in the presence of G. lucidium is attributed to this effect. This study potentially amplifies the scope of the use of G. lucidum, which was earlier shown to have only reactive oxygen species (ROS) scavenging properties with regards to DNA damage inhibition.

MeSH terms

  • DNA Breaks, Double-Stranded / drug effects
  • DNA Breaks, Single-Stranded / drug effects
  • DNA Damage / drug effects*
  • DNA Repair / drug effects
  • DNA-(Apurinic or Apyrimidinic Site) Lyase / drug effects
  • DNA-(Apurinic or Apyrimidinic Site) Lyase / genetics*
  • Fibroblasts / drug effects*
  • Fungal Polysaccharides / administration & dosage*
  • Fungal Polysaccharides / chemistry
  • Humans
  • Nucleic Acid Conformation / drug effects
  • Reactive Oxygen Species / metabolism
  • Reishi / chemistry

Substances

  • Fungal Polysaccharides
  • Reactive Oxygen Species
  • APEX1 protein, human
  • DNA-(Apurinic or Apyrimidinic Site) Lyase