A cell-surface-anchored ratiometric i-motif sensor for extracellular pH detection

Le Ying; Nuli Xie; Yanjing Yang; Xiaohai Yang; Qifeng Zhou; Bincheng Yin; Jin Huang; Kemin Wang

doi:10.1039/c6cc03163c

A cell-surface-anchored ratiometric i-motif sensor for extracellular pH detection

Chem Commun (Camb). 2016 Jun 14;52(50):7818-21. doi: 10.1039/c6cc03163c.

Authors

Le Ying¹, Nuli Xie¹, Yanjing Yang¹, Xiaohai Yang¹, Qifeng Zhou¹, Bincheng Yin², Jin Huang¹, Kemin Wang¹

Affiliations

¹ State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha, China. jinhuang@hnu.edu.cn kmwang@hnu.edu.cn.
² State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.

PMID: 27241716
DOI: 10.1039/c6cc03163c

Abstract

A FRET-based sensor is anchored on the cell surface through streptavidin-biotin interactions. Due to the excellent properties of the pH-sensitive i-motif structure, the sensor can detect extracellular pH with high sensitivity and excellent reversibility.

MeSH terms

Biotin / chemistry
Cell Line
Cell Membrane / chemistry
Fluorescence Resonance Energy Transfer / methods*
Fluorescent Dyes / analysis*
Fluorescent Dyes / chemistry*
Humans
Hydrogen-Ion Concentration
Streptavidin / chemistry
Surface Properties

Substances

Fluorescent Dyes
Biotin
Streptavidin