Selective immunoprecipitation of proteins is a useful tool for characterizing proteins and protein-protein interactions. Clear step-by-step protocols are provided for preparing lysates of cells and yeast under a variety of conditions, for binding the antibody to a solid matrix, and for performing the actual immunoprecipitation. An additional method is provided for increasing the specificity of the technique by reprecipitating the antigen with the same or a different antibody. © 2016 by John Wiley & Sons, Inc.
Keywords: agarose bead; antibody; antigen; immunoprecipitation; immunoprecipitation-recapture; magnetic bead.
Copyright © 2016 John Wiley & Sons, Inc.