Assay of Endocannabinoid Uptake

Methods Mol Biol. 2016;1412:191-203. doi: 10.1007/978-1-4939-3539-0_20.

Abstract

Endocannabinoids at physiological concentrations are crossing cellular membranes by facilitated diffusion, a process that can be studied by measuring transport kinetics. Here, we describe a radiosubstrate-based assay using arachidonoyl[1-(3)H]ethanolamine or arachidonoyl[1,2,3-(3)H]glycerol to measure the cellular endocannabinoid uptake in a three-phase assay with human U937 cells.

Keywords: 2-Arachidonoylglycerol; Anandamide; Endocannabinoid uptake.

MeSH terms

  • Biological Assay*
  • Cell Membrane / metabolism
  • Endocannabinoids / metabolism*
  • Humans
  • Hydrolysis
  • Radiometry / methods
  • U937 Cells

Substances

  • Endocannabinoids