Co-culture of Gastric Organoids and Immortalized Stomach Mesenchymal Cells

Methods Mol Biol. 2016;1422:23-31. doi: 10.1007/978-1-4939-3603-8_3.


Three-dimensional primary epithelial-derived gastric organoids have recently been established as an important tool to study gastric development, physiology, and disease. Specifically, mouse-derived fundic gastric organoids (mFGOs) co-cultured with Immortalized Stomach Mesenchymal Cells (ISMCs) reflect expression patterns of mature fundic cell types seen in vivo, thus allowing for long-term in vitro studies of gastric epithelial cell physiology, regeneration, and bacterial-host interactions. Here, we describe the development and culture of mFGOs, co-cultured with ISMCs.

Keywords: Co-culture; Immortalized stomach mesenchymal cells; Mouse fundic gastric organoids.

MeSH terms

  • Animals
  • Biomarkers / metabolism
  • Cell Proliferation
  • Cells, Cultured
  • Coculture Techniques / methods*
  • Epithelial Cells / cytology
  • Epithelial Cells / metabolism
  • Gastric Mucosa / cytology*
  • Gastric Mucosa / metabolism
  • Mesenchymal Stem Cells / cytology*
  • Mesenchymal Stem Cells / metabolism
  • Mice
  • Organoids / cytology*
  • Organoids / metabolism


  • Biomarkers