Paf15 expression correlates with rectal cancer prognosis, cell proliferation and radiation response

Oncotarget. 2016 Jun 21;7(25):38750-38761. doi: 10.18632/oncotarget.9606.


Paf15, which participates in DNA repair, is overexpressed in numerous solid tumors. Blocking of Paf15 inhibits the growth of many types of cancer cells; while simultaneously enhancing cellular sensitivity to UV radiation. However, its expression and function in rectal cancer (RC) remain unknown. The current study was undertaken to assess the association of Paf15 expression with RC prognosis, as well as to explore the participation of Paf15 in the response of RC cells to irradiation. Increased Paf15 expression was observed in RC tissues and associated with pTNM stage and poor survival. In vitro, Paf15 induced increased RC cell proliferation while accelerating cell cycle progression, inhibiting cell death, and protecting against gamma radiation-induced DNA damage in RC cells. In conclusion, increased Paf15 expression is associated with increased RC proliferation, decreased patient survival, and a worse radiotherapeutic response.

Keywords: Paf15; cell cycle; gamma radiation; prognostic factor; rectal cancer.

MeSH terms

  • Aged
  • Carrier Proteins / metabolism*
  • Cell Cycle
  • Cell Death
  • Cell Line, Tumor
  • Cell Proliferation
  • Cell Survival
  • DNA Damage
  • DNA Repair*
  • DNA-Binding Proteins
  • Female
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Male
  • Middle Aged
  • Prognosis
  • Proportional Hazards Models
  • Rectal Neoplasms / pathology*
  • Rectal Neoplasms / radiotherapy
  • Treatment Outcome


  • Carrier Proteins
  • DNA-Binding Proteins
  • PCLAF protein, human