Long-Term Potentiation by Theta-Burst Stimulation Using Extracellular Field Potential Recordings in Acute Hippocampal Slices

Therese Abrahamsson; Txomin Lalanne; Alanna J Watt; P Jesper Sjöström

doi:10.1101/pdb.prot091298

Long-Term Potentiation by Theta-Burst Stimulation Using Extracellular Field Potential Recordings in Acute Hippocampal Slices

Cold Spring Harb Protoc. 2016 Jun 1;2016(6):pdb.prot091298. doi: 10.1101/pdb.prot091298.

Authors

Therese Abrahamsson¹, Txomin Lalanne², Alanna J Watt³, P Jesper Sjöström¹

Affiliations

¹ Centre for Research in Neuroscience, Department of Neurology and Neurosurgery, The Research Institute of the McGill University Health Centre, Montréal General Hospital, Montréal, Québec H3G 1A4, Canada;
² Centre for Research in Neuroscience, Department of Neurology and Neurosurgery, The Research Institute of the McGill University Health Centre, Montréal General Hospital, Montréal, Québec H3G 1A4, Canada; Integrated Program in Neuroscience, McGill University, Montréal, Quebec H3A 2B4, Canada;
³ Department of Biology, Bellini Life Sciences Building, McGill University, Montréal, Québec H3G 0B1, Canada.

Abstract

This protocol describes how to carry out theta-burst long-term potentiation (LTP) with extracellular field recordings in acute rodent hippocampal slices. This method is relatively simple and noninvasive and provides a way to sample many neurons simultaneously, making it suitable for applications requiring higher throughput than whole-cell recording.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Hippocampus / physiology*
In Vitro Techniques / methods*
Long-Term Potentiation*
Patch-Clamp Techniques / methods*
Rodentia

Abstract

Publication types

MeSH terms

Grants and funding