Compartment-Specific Regulation of Autophagy in Primary Neurons

J Neurosci. 2016 Jun 1;36(22):5933-45. doi: 10.1523/JNEUROSCI.4401-15.2016.

Abstract

Autophagy is an essential degradative pathway that maintains neuronal homeostasis and prevents axon degeneration. Initial observations suggest that autophagy is spatially regulated in neurons, but how autophagy is regulated in distinct neuronal compartments is unclear. Using live-cell imaging in mouse hippocampal neurons, we establish the compartment-specific mechanisms of constitutive autophagy under basal conditions, as well as in response to stress induced by nutrient deprivation. We find that at steady state, the cell soma contains populations of autophagosomes derived from distinct neuronal compartments and defined by differences in maturation state and dynamics. Axonal autophagosomes enter the soma and remain confined within the somatodendritic domain. This compartmentalization likely facilitates cargo degradation by enabling fusion with proteolytically active lysosomes enriched in the soma. In contrast, autophagosomes generated within the soma are less mobile and tend to cluster. Surprisingly, starvation did not induce autophagy in either the axonal or somatodendritic compartment. While starvation robustly decreased mTORC1 signaling in neurons, this decrease was not sufficient to activate autophagy. Furthermore, pharmacological inhibition of mammalian target of rapamycin with Torin1 also was not sufficient to markedly upregulate neuronal autophagy. These observations suggest that the primary physiological function of autophagy in neurons may not be to mobilize amino acids and other biosynthetic building blocks in response to starvation, in contrast to findings in other cell types. Rather, constitutive autophagy in neurons may function to maintain cellular homeostasis by balancing synthesis and degradation, especially within distal axonal processes far removed from the soma.

Significance statement: Autophagy is an essential homeostatic process in neurons, but neuron-specific mechanisms are poorly understood. Here, we compare autophagosome dynamics within neuronal compartments. Axonal autophagy is a vectorial process that delivers cargo from the distal axon to the soma. The soma, however, contains autophagosomes at different maturation states, including input received from the axon combined with locally generated autophagosomes. Once in the soma, autophagosomes are confined to the somatodendritic domain, facilitating cargo degradation and recycling of biosynthetic building blocks to primary sites of protein synthesis. Neuronal autophagy is not robustly upregulated in response to starvation or mammalian target of rapamycin inhibition, suggesting that constitutive autophagy in neurons maintains homeostasis by playing an integral role in regulating the quality of the neuronal proteome.

Keywords: LC3; autophagy; axonal transport; hippocampal neuron; mTOR; soma.

MeSH terms

  • Amino Acids / deficiency
  • Animals
  • Autophagy / drug effects
  • Autophagy / physiology*
  • Axons / drug effects
  • Axons / physiology
  • Biological Transport / drug effects
  • Biological Transport / physiology
  • Cells, Cultured
  • Dendrites / drug effects
  • Dendrites / physiology
  • Embryo, Mammalian
  • Enzyme Inhibitors / toxicity
  • Female
  • Hippocampus / cytology
  • Homeostasis / drug effects
  • Luminescent Proteins / genetics
  • Luminescent Proteins / metabolism
  • Lysosome-Associated Membrane Glycoproteins / genetics
  • Lysosome-Associated Membrane Glycoproteins / metabolism
  • Macrolides / toxicity
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Microtubule-Associated Proteins / genetics
  • Microtubule-Associated Proteins / metabolism
  • Naphthyridines / toxicity
  • Neurons / cytology*
  • Neurons / drug effects
  • Neurons / physiology*
  • Phagosomes / metabolism

Substances

  • 1-(4-(4-propionylpiperazin-1-yl)-3-(trifluoromethyl)phenyl)-9-(quinolin-3-yl)benzo(h)(1,6)naphthyridin-2(1H)-one
  • Amino Acids
  • Enzyme Inhibitors
  • Lamp1 protein, mouse
  • Luminescent Proteins
  • Lysosome-Associated Membrane Glycoproteins
  • Macrolides
  • Map1lc3b protein, mouse
  • Microtubule-Associated Proteins
  • Naphthyridines
  • bafilomycin A1