The aim of this study was to evaluate shear bond strength (SBS) and microleakage of a self-etching adhesive (Adper Prompt L-Pop) in comparison to acid etching prior fissure sealing. Each procedure was tested with 3 aging procedures (1-day water storage, 3-month water storage and 1-day water storage/5,000× thermocycling). SBS was determined according to ISO standard 29022. Additional 30 third molars were utilized for the microleakage analyses. Data were analyzed using Mann-Whitney-U-Test and multiple linear regression models. The SBS of the self-etching adhesive were significantly lower (14.9, 11.9, and 13.0 MPa) than those of conventional fissure sealing (19.1, 18.2, and 15.6 MPa). Multiple linear regression models predicted that material and alteration significantly influenced SBS. The microleakage revealed no difference between both groups (1.3% vs. 1.2%). It can be concluded that the selfetching adhesive might be a pre-treatment alternative for fissure sealing in terms of the easier and shorter clinical workflow.