[Anti-proliferation Effect of Taraxacum mongolicum Extract in HepG2 Cells and Its Mechanism]

Zhong Yao Cai. 2015 Oct;38(10):2129-33.
[Article in Chinese]

Abstract

Objective: To study the anti-proliferation effect of Taraxacum mongolicum extract in HepG2 cells and its mechanism.

Methods: The total proteins of HepG2 cells treated with Taraxacum mongolicum extract were. extracted and mitochondria-mediated apoptosis-related proteins (Survivin, Mcl-1, BCL-xL, BCL-2, Smac, BAX, Bad, Cytochrome c and Caspase-3/7/9) were detected by Western blot.

Results: Taraxacum mongolicum extract obviously inhibited the proliferation of HepG2 cells and the expression of anti-apoptotic proteins (Survivin, BCL-xL and BCL-2), increased the expression of pro-apoptotic proteins (Smac and Caspase-3/7/9), and promoted the release of Cytochrome c from mitochondria to cytoplasm in HepG2 cells. The effects were in a dose-independent mode.

Conclusion: Taraxacum mongolicum extract can inhibit the proliferation of HepG2 cells and the anti-proliferation mechanism is related to mitochondria-mediated apoptosis.

MeSH terms

  • Apoptosis Regulatory Proteins / metabolism
  • Apoptosis*
  • Caspases / metabolism
  • Cell Proliferation / drug effects
  • Cytochromes c / metabolism
  • Hep G2 Cells / drug effects
  • Humans
  • Mitochondria / metabolism*
  • Plant Extracts / pharmacology*
  • Taraxacum / chemistry*

Substances

  • Apoptosis Regulatory Proteins
  • Plant Extracts
  • Cytochromes c
  • Caspases