Roles of RNA-binding Proteins and Post-transcriptional Regulation in Driving Male Germ Cell Development in the Mouse

Adv Exp Med Biol. 2016;907:123-51. doi: 10.1007/978-3-319-29073-7_6.


Tissue development and homeostasis are dependent on highly regulated gene expression programs in which cell-specific combinations of regulatory factors determine which genes are expressed and the post-transcriptional fate of the resulting RNA transcripts. Post-transcriptional regulation of gene expression by RNA-binding proteins has critical roles in tissue development-allowing individual genes to generate multiple RNA and protein products, and the timing, location, and abundance of protein synthesis to be finely controlled. Extensive post-transcriptional regulation occurs during mammalian gametogenesis, including high levels of alternative mRNA expression, stage-specific expression of mRNA variants, broad translational repression, and stage-specific activation of mRNA translation. In this chapter, an overview of the roles of RNA-binding proteins and the importance of post-transcriptional regulation in male germ cell development in the mouse is presented.

Keywords: Alternative mRNA processing; Gametogenesis; Polyadenylation; Post-transcriptional regulation; RNA-binding proteins; Splicing; Translational control.

MeSH terms

  • Animals
  • Gene Expression Regulation, Developmental*
  • Germ Cells / cytology*
  • Humans
  • Male
  • Meiosis
  • Mice
  • Protein Biosynthesis
  • RNA / genetics*
  • RNA / metabolism
  • RNA Processing, Post-Transcriptional*
  • RNA Splicing
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • RNA-Binding Proteins / physiology*
  • Sertoli Cells / physiology
  • Species Specificity
  • Spermatogenesis / physiology*
  • Testis / embryology


  • RNA, Messenger
  • RNA-Binding Proteins
  • RNA