Abstract
In reconstitution studies N-ethylmaleimide, a sulphydryl alkylating reagent, inhibits both fusion of endocytic vesicles and vesicular transport in the Golgi apparatus. We show here that the same N-ethylmaleimide-sensitive factor that catalyses the vesicle-mediated transport within Golgi stacks is also required for endocytic vesicle fusion. Thus, it is likely that a common mechanism for vesicle fusion exists for both the secretory and endocytic pathways of eukaryotic cells.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antibodies, Monoclonal
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Antigen-Antibody Complex
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Carrier Proteins / physiology*
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Cell Line
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Cytosol / metabolism
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Endocytosis*
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Ethylmaleimide / pharmacology*
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Golgi Apparatus / metabolism*
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Immune Sera
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Kinetics
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N-Ethylmaleimide-Sensitive Proteins
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Vesicular Transport Proteins*
Substances
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Antibodies, Monoclonal
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Antigen-Antibody Complex
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Carrier Proteins
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Immune Sera
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Vesicular Transport Proteins
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N-Ethylmaleimide-Sensitive Proteins
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Ethylmaleimide