Vesicle fusion following receptor-mediated endocytosis requires a protein active in Golgi transport

Nature. 1989 Jun 1;339(6223):398-400. doi: 10.1038/339398a0.

Abstract

In reconstitution studies N-ethylmaleimide, a sulphydryl alkylating reagent, inhibits both fusion of endocytic vesicles and vesicular transport in the Golgi apparatus. We show here that the same N-ethylmaleimide-sensitive factor that catalyses the vesicle-mediated transport within Golgi stacks is also required for endocytic vesicle fusion. Thus, it is likely that a common mechanism for vesicle fusion exists for both the secretory and endocytic pathways of eukaryotic cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Antigen-Antibody Complex
  • Carrier Proteins / physiology*
  • Cell Line
  • Cytosol / metabolism
  • Endocytosis*
  • Ethylmaleimide / pharmacology*
  • Golgi Apparatus / metabolism*
  • Immune Sera
  • Kinetics
  • N-Ethylmaleimide-Sensitive Proteins
  • Vesicular Transport Proteins*

Substances

  • Antibodies, Monoclonal
  • Antigen-Antibody Complex
  • Carrier Proteins
  • Immune Sera
  • Vesicular Transport Proteins
  • N-Ethylmaleimide-Sensitive Proteins
  • Ethylmaleimide