Cloning of cDNA for the Major DNA-binding Protein of the Erythroid Lineage Through Expression in Mammalian Cells

Nature. 1989 Jun 8;339(6224):446-51. doi: 10.1038/339446a0.

Abstract

Genes expressed in erythroid cells contain binding sites for a cell-specific factor believed to be an important regulator for this haematopoietic lineage. Using high-level transient expression in mammalian cells, we have identified complementary DNA encoding the murine protein. The factor, a new member of the zinc-finger family of DNA-binding proteins, is restricted to erythroid cells at the level of RNA expression and is closely homologous between mouse and man.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA / genetics*
  • DNA-Binding Proteins / blood
  • DNA-Binding Proteins / genetics*
  • Erythrocytes / metabolism*
  • Erythroid-Specific DNA-Binding Factors
  • Genes*
  • Humans
  • Mice
  • Molecular Sequence Data
  • RNA, Messenger / genetics
  • Transcription Factors*
  • Transcription, Genetic

Substances

  • DNA-Binding Proteins
  • Erythroid-Specific DNA-Binding Factors
  • RNA, Messenger
  • Transcription Factors
  • DNA