Effect of Molybdenum Nanoparticles on Blood Cells, Liver Enzymes, and Sexual Hormones in Male Rats

Biol Trace Elem Res. 2017 Jan;175(1):50-56. doi: 10.1007/s12011-016-0765-5. Epub 2016 Jun 4.


Despite an increasing surge in application of nanoparticles in industries, there is a serious lack of information concerning their impact on human health and the environment. The present study investigated effects of molybdenum nanoparticles (Mo NPs) injected intraperitoneally into Sprague-Dawley rats at different doses of Mo NPs (5, 10, and 15 mg/kg BW per day) during a period of 28 days. Hematological and biochemical parameters as well as sexual hormones and histopathological examinations of the liver and testis were assessed and compared with control group. The results showed that the serum levels of testosterone decreased significantly in both groups of 10 and 15 mg (Mo NPs)/kg BW in comparison with the control group (p < 0.05). However, there were insignificant differences observed in luteinizing hormone (LH) levels and hematological parameters when compared with the control group (p > 0.05). The results of liver enzymes showed that serum levels of aspartate aminotransferase (AST) decreased significantly in both dosage groups of 5 and 10 mg/kg BW (Mo NPs) when compared with the control group (p < 0.05), and significant decrease obtained in lactate dehydrogenase (LDH) levels at dose of 5 mg/kg BW in comparison with the control group (p < 0.05). The histopathological examination of testis showed a decrease in number of Leydig cells. Also, the number of chronic inflammatory cells increased in portal triad and parenchyma in liver tissue of rats exposed to Mo NPs.

Keywords: Hormone; Liver; Molybdenum nanoparticles; Pathology; Testis.

MeSH terms

  • Animals
  • Aspartate Aminotransferases / blood*
  • Blood Cells / metabolism*
  • Humans
  • L-Lactate Dehydrogenase / blood*
  • Leydig Cells / metabolism
  • Liver / enzymology*
  • Luteinizing Hormone / blood*
  • Male
  • Metal Nanoparticles / toxicity*
  • Molybdenum*
  • Rats
  • Rats, Sprague-Dawley
  • Testosterone / blood*


  • Testosterone
  • Molybdenum
  • Luteinizing Hormone
  • L-Lactate Dehydrogenase
  • Aspartate Aminotransferases