Cissus quadrangularis prevented the ovariectomy induced oxidative stress in the femur of adult albino rats

Biomed Pharmacother. 2016 Jul;81:416-423. doi: 10.1016/j.biopha.2016.04.021. Epub 2016 Apr 28.


The increasing evidence suggesting the role of free radicals in bone resorption and bone loss prompted us to explore whether the consumption of antioxidant rich medicinal plant C. quadrangularis modifies antioxidant status in ovariectomized rats.

Methods: Twenty four female adult rats, 90days old showing regular estrous cycles were used for the present study. The animals were divided into two groups. The Group-1 rats (n=6) were sham operated and Group-II rats were bilaterally ovariectomized (n=18) and treated with C. quadrangularis for sixty days (100mg/kg body weight and 250mg/kg body weight). After sixty days, the rats were killed, femora were dissected out, minced and homogenized in Tris-HCl buffer (pH 7.4) and the supernatant was collected and used for biochemical assays.

Results: Ovariectomy registered a decrease (p<0.05) in the activities of SOD, GPx, GST, ALP, collagen content and increased (p<0.05) the activities of TRAP and lipid peroxidation. Simultaneous administration of C. quadrangularis maintained the enzyme activities in ovariectomized rats.

Conclusion: C. quadrangularis, a natural herb may be used to treat the estrogen deficiency/menopause onset and ovariectomy induced oxidative stress.

Keywords: Bone loss; C. quadrangularis; Ovariectomy; Oxidative stress.

MeSH terms

  • Aging
  • Alkaline Phosphatase / metabolism
  • Animals
  • Cissus / chemistry*
  • Collagen / metabolism
  • Female
  • Femur / drug effects*
  • Femur / enzymology
  • Femur / pathology*
  • Glutathione Peroxidase / metabolism
  • Glutathione Transferase / metabolism
  • Hydrogen Peroxide / metabolism
  • Lipid Peroxidation / drug effects
  • Ovariectomy / adverse effects*
  • Oxidative Stress / drug effects*
  • Plant Extracts / pharmacology*
  • Rats, Wistar
  • Superoxide Dismutase / metabolism
  • Tartrate-Resistant Acid Phosphatase / metabolism


  • Plant Extracts
  • Collagen
  • Hydrogen Peroxide
  • Glutathione Peroxidase
  • Superoxide Dismutase
  • Glutathione Transferase
  • Alkaline Phosphatase
  • Tartrate-Resistant Acid Phosphatase