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. 2016 Sep;38(9):1443-8.
doi: 10.1007/s10529-016-2142-z. Epub 2016 Jun 4.

Enterococcus Faecalis Promotes Osteoclast Differentiation Within an Osteoblast/Osteoclast Co-Culture System

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Enterococcus Faecalis Promotes Osteoclast Differentiation Within an Osteoblast/Osteoclast Co-Culture System

Zuhui Deng et al. Biotechnol Lett. .

Abstract

Objective: To investigate the effects of heat-killed Enterococcus faecalis ATCC 29212 and P25RC clinical strain (derived from an obturated root canal with apical periodontitis) on osteoclast differentiation within an osteoblast/osteoclast co-culture system.

Results: Heat-killed E. faecalis significantly increased the proportion of multinucleated osteoclastic cells (MNCs) within the co-culture system. The IL-6 level was significantly increased upon exposure to heat-killed E. faecalis. Gene expression levels of NFATc1 and cathepsin K were significantly up-regulated compared to the untreated control. EphrinB2 and EphB4 expressions at both the mRNA and protein levels were also significantly upregulated compared to the untreated control.

Conclusions: Heat-killed E. faecalis can induce osteoclast differentiation within the osteoblast/osteoclast co-culture system in vitro, possibly through ephrinB2-EphB4 bidirectional signaling.

Keywords: Enterococcus faecalis; EphB4; EphrinB2; Multinucleated osteoclastic cells; Osteoblasts; Osteoclast differentiation; Osteoclasts.

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