Epidermal growth factor-induced hydrogen peroxide production is mediated by dual oxidase 1

Free Radic Biol Med. 2016 Aug;97:204-211. doi: 10.1016/j.freeradbiomed.2016.05.028. Epub 2016 Jun 1.


Stimulation of mammalian cells by epidermal growth factor (EGF) elicits complex signaling events, including an increase in hydrogen peroxide (H2O2) production. Understanding the significance of this response is limited by the fact that the source of EGF-induced H2O2 production is unknown. Here we show that EGF-induced H2O2 production in epidermal cell lines is dependent on the agonist-induced calcium signal. We analyzed the expression of NADPH oxidase isoforms and found both A431 and HaCaT cells to express the calcium-sensitive NADPH oxidase, Dual oxidase 1 (Duox1) and its protein partner Duox activator 1 (DuoxA1). Inhibition of Duox1 expression by small interfering RNAs eliminated EGF-induced H2O2 production in both cell lines. We also demonstrate that H2O2 production by Duox1 leads to the oxidation of thioredoxin-1 and the cytosolic peroxiredoxins. Our observations provide evidence for a new signaling paradigm in which changes of intracellular calcium concentration are transformed into redox signals through the calcium-dependent activation of Duox1.

Keywords: Dual oxidase 1; EGF receptor; Hydrogen peroxide; NADPH oxidase; Reactive oxygen species.

MeSH terms

  • Animals
  • Calcium / metabolism
  • Calcium Signaling / genetics
  • Cytosol / metabolism
  • Dual Oxidases / genetics
  • Dual Oxidases / metabolism*
  • ErbB Receptors / genetics*
  • ErbB Receptors / metabolism
  • Humans
  • Hydrogen Peroxide / metabolism*
  • NADPH Oxidases / genetics
  • NADPH Oxidases / metabolism*
  • Oxidation-Reduction
  • Protein Isoforms / metabolism
  • RNA, Small Interfering
  • Reactive Oxygen Species / metabolism
  • Signal Transduction
  • Thioredoxins / genetics
  • Thioredoxins / metabolism


  • Protein Isoforms
  • RNA, Small Interfering
  • Reactive Oxygen Species
  • Thioredoxins
  • Hydrogen Peroxide
  • Dual Oxidases
  • NADPH Oxidases
  • DUOX1 protein, human
  • EGFR protein, human
  • ErbB Receptors
  • Calcium