An influence of the copy number of biosynthetic gene clusters on the production level of antibiotics in a heterologous host

J Biotechnol. 2016 Aug 20:232:110-7. doi: 10.1016/j.jbiotec.2016.05.038. Epub 2016 Jun 2.

Abstract

Streptomyces albus J1074 is a well-known host for heterologous expression of secondary metabolites. To further increase its potential and to study the influence of cluster multiplication, additional φC31-attachment site was integrated into its genome using a system for transposon mutagenesis. Four secondary metabolite clusters were expressed in strains with different numbers of attachment sites, ranging from one to three copies of the site. Secondary metabolite production was examined and a new compound could be detected, purified and its structure was elucidated.

Keywords: Antibiotic production; Heterologous expression; Multiple attachment sites; Strain development; Streptomyces albus J1074; φC31-encoded recombination system.

MeSH terms

  • Anti-Bacterial Agents / analysis
  • Anti-Bacterial Agents / metabolism*
  • Gene Dosage / genetics*
  • Genes, Bacterial / genetics*
  • Multigene Family / genetics*
  • Plasmids / genetics
  • Streptomyces / genetics*
  • Streptomyces / metabolism*

Substances

  • Anti-Bacterial Agents